3D Micropillars Guide the Mechanobiology of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Raghavendra Palankar; Michael Glaubitz; Ulrike Martens; Nikolay Medvedev; Marvin von der Ehe; Stephan B Felix; Markus Münzenberg; Mihaela Delcea

doi:10.1002/adhm.201500740

3D Micropillars Guide the Mechanobiology of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Adv Healthc Mater. 2016 Feb 4;5(3):335-41. doi: 10.1002/adhm.201500740. Epub 2015 Dec 16.

Authors

Raghavendra Palankar¹, Michael Glaubitz¹, Ulrike Martens², Nikolay Medvedev¹, Marvin von der Ehe², Stephan B Felix^{3

4}, Markus Münzenberg², Mihaela Delcea^{1

4}

Affiliations

¹ ZIK HIKE - Centre for Innovation Competence (Humoral Immune Reactions in Cardiovascular Diseases), Ernst-Moritz-Arndt-University, 17489, Greifswald, Germany.
² Institute for Physics, University of Greifswald, 17489, Greifswald, Germany.
³ Clinic for Internal Medicine B (Cardiology), University of Greifswald Sauebruchstrasse, 17475, Greifswald, Germany.
⁴ DZHK (German Centre for Cardiovascular Research) partner site, Greifswald, Germany.

PMID: 26676091
DOI: 10.1002/adhm.201500740

Abstract

3D micropillars generated by photolithography are used as a platform to probe by atomic force microscopy the mechanodynamics of human induced pluripotent stem cell-derived cardiomyocytes. 3D micropillars guide subcellular cytoskeletal modifications of cardiomyocytes and lead to biochemical changes altering beating rate, stiffness, and calcium dynamics of the cells.

Keywords: 3D micropillars; atomic force microscopy; induced pluripotent stem cells; mechanobiology; sarcomeres.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calcium / metabolism
Cell Differentiation / physiology
Cells, Cultured
Humans
Induced Pluripotent Stem Cells / metabolism
Induced Pluripotent Stem Cells / physiology*
Myocytes, Cardiac / metabolism
Myocytes, Cardiac / physiology*

Substances

Calcium