Blood levels of serotonin are specifically correlated with plasma lysophosphatidylserine among the glycero-lysophospholipids

Makoto Kurano; Tomotaka Dohi; Takahiro Nojiri; Tamaki Kobayashi; Yuji Hirowatari; Asuka Inoue; Kuniyuki Kano; Hirotaka Matsumoto; Koji Igarashi; Masako Nishikawa; Katsumi Miyauchi; Hiroyuki Daida; Hitoshi Ikeda; Junken Aoki; Yutaka Yatomi

doi:10.1016/j.bbacli.2015.08.003

Blood levels of serotonin are specifically correlated with plasma lysophosphatidylserine among the glycero-lysophospholipids

BBA Clin. 2015 Aug 20:4:92-8. doi: 10.1016/j.bbacli.2015.08.003. eCollection 2015 Dec.

Authors

Affiliations

¹ The University of Tokyo, Department of Clinical Laboratory Medicine, Graduate School of Medicine, Tokyo, Japan ; CREST, Japan Science and Technology Corporation (JST), Japan.
² Juntendo University School of Medicine, Department of Cardiovascular Medicine, Japan.
³ The University of Tokyo Hospital, Department of Clinical Laboratory, Tokyo, Japan.
⁴ Bioscience Division, TOSOH Corporation, Kanagawa, Japan ; Saitama Prefectural University, Laboratory Science, Department of Health Science, Saitama, Japan.
⁵ Tohoku University, Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Miyagi, Japan ; PRESTO, Japan Science and Technology Corporation (JST), Japan.
⁶ CREST, Japan Science and Technology Corporation (JST), Japan ; Tohoku University, Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Miyagi, Japan.
⁷ Tohoku University, Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Miyagi, Japan.
⁸ Bioscience Division, TOSOH Corporation, Kanagawa, Japan.
⁹ The University of Tokyo, Department of Clinical Laboratory Medicine, Graduate School of Medicine, Tokyo, Japan ; CREST, Japan Science and Technology Corporation (JST), Japan ; The University of Tokyo Hospital, Department of Clinical Laboratory, Tokyo, Japan.

Abstract

Backgrounds: Glycero-lysophospholipids (glycero-LPLs), which are known to exert potent biological activities, have been demonstrated to be secreted from activated platelets in vitro; however, their association with platelet activation in vivo has not been yet elucidated. In this study, we investigated the correlations between the blood levels of each glycero-LPL and serotonin, a biomarker of platelet activation, in human subjects to elucidate the involvement of platelet activation in glycero-LPLs in vivo.

Methods and results: We measured the plasma serotonin levels in 141 consecutive patients undergoing coronary angiography (acute coronary syndrome, n = 38; stable angina pectoris, n = 71; angiographically normal coronary arteries, n = 32) and investigated the correlations between the plasma levels of serotonin and glycero-LPLs. The results revealed the existence of a specific and significant association between the plasma serotonin and plasma lysophosphatidylserine (LysoPS) levels. On the contrary, regular aspirin intake failed to affect the plasma LysoPS levels despite the fact that the plasma lysophosphatidic acid, lysophosphatidylethanolamine, lysophosphatidylglycerol, and lysophosphatidylinositol levels were lower in those who had taken aspirin regularly.

Conclusion: We found a specific positive correlation between the blood levels of serotonin and LysoPS, a new lipid mediator. Thus, LysoPS might be specifically involved in strong platelet activation, which is associated with the release of serotonin.

General significance: Our present results suggest the possible involvement of LysoPS in the pathogenesis of atherosclerotic diseases.

Keywords: ACS, acute coronary syndrome; Acute coronary syndrome; Aspirin; Glycero-LPL, glycero-lysophospholipid; Glycero-lysophospholipids; LC-MS/MS, liquid chromatography-tandem mass spectrometry; LPL, lysophospholipid; LysoPA, lysophosphatidic acids; LysoPC, lysophosphatidylcholine; LysoPE, lysophosphatidylethanolamine; LysoPG, lysophosphatidylglycerol; LysoPI, lysophosphatidylinositol; LysoPS, lysophosphatidylserine; Lysophosphatidylserine; NCA, angiographically normal coronary arteries; PS, phosphatidylserine; PS-PLA1, phosphatidylserine-specific phospholipase A1;; SAP, stable angina pectoris; Serotonin.