Abstract
We compared the effect of alpha-tocopheryl succinate (TOS) on succinate-dependent respiration in rat liver mitochondria, homogenate and permeabilized hepatocytes in both a coupled and uncoupled state. In isolated mitochondria, a significant inhibitory effect was observed at a concentration of 5 microM, in liver homogenate at 25 microM and in permeabilized hepatocytes at 50 microM. The inhibitory effect of TOS on succinate respiration in an uncoupled state was less pronounced than in a coupled state in all the experimental models tested. When the concentration dependence of the TOS inhibitory effect was tested, the most sensitive in both states were isolated mitochondria; the most resistant were permeabilized hepatocytes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / pharmacology
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Cell Membrane / metabolism
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Cell Membrane Permeability
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Cell Respiration / drug effects
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Dose-Response Relationship, Drug
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Electron Transport Complex II / metabolism
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Energy Metabolism / drug effects*
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Hepatocytes / drug effects*
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Hepatocytes / metabolism
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Male
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Mitochondria, Liver / drug effects*
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Mitochondria, Liver / metabolism
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Oxidative Phosphorylation / drug effects
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Rats, Wistar
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Time Factors
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Uncoupling Agents / pharmacology
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alpha-Tocopherol / metabolism
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alpha-Tocopherol / pharmacology*
Substances
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Uncoupling Agents
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Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
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Electron Transport Complex II
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alpha-Tocopherol