High-yield production of canine parvovirus virus-like particles in a baculovirus expression system

Arch Virol. 2016 Mar;161(3):705-10. doi: 10.1007/s00705-015-2719-1. Epub 2015 Dec 14.

Abstract

An optimized VP2 gene from the current prevalent CPV strain (new CPV-2a) in China was expressed in a baculovirus expression system. It was found that the VP2 proteins assembled into virus-like particles (VLPs) with antigenic properties similar to those of natural CPV and with an especially high hemagglutination (HA) titer (1:2(20)). Dogs intramuscularly or orally immunized with VLPs produced antibodies against CPV with >1:80 hemagglutination inhibition (HI) units for at least 3 months. The CPV VLPs could be considered for use as a vaccine against CPV or as a platform for research on chimeric VLP vaccines against other diseases.

Keywords: Canine parvovirus; Vaccine; Virus-like particles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Antibodies, Viral / blood
  • Baculoviridae*
  • China
  • Dogs
  • Genetic Vectors*
  • Injections, Intramuscular
  • Parvovirus, Canine / genetics*
  • Protein Multimerization
  • Vaccines, Virosome / administration & dosage
  • Vaccines, Virosome / genetics
  • Vaccines, Virosome / immunology
  • Viral Structural Proteins / genetics
  • Viral Structural Proteins / metabolism*
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / genetics
  • Viral Vaccines / immunology
  • Virosomes / genetics
  • Virosomes / metabolism*

Substances

  • Antibodies, Viral
  • Vaccines, Virosome
  • Viral Structural Proteins
  • Viral Vaccines
  • Virosomes