Fusogenic Reactive Oxygen Species Triggered Charge-Reversal Vector for Effective Gene Delivery

Adv Mater. 2016 Mar 2;28(9):1743-52. doi: 10.1002/adma.201504288. Epub 2015 Dec 14.

Abstract

A novel fusogenic lipidic polyplex (FLPP) vector is designed to fuse with cell membranes, mimicking viropexis, and eject the polyplex into the cytosol, where the cationic polymer is subsequently oxidized by intracellular reactive oxygen species and converts to being negatively charged, efficiently releasing the DNA. The vector delivering suicide gene achieves significantly better inhibition of tumor growth than doxorubicin.

Keywords: ROS-responsive; charge reversal polymers; fusogenic lipid vectors; gene delivery; phenylboronic acid (PBA).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Transport
  • Cell Line, Tumor
  • DNA / chemistry
  • DNA / genetics
  • Drug Carriers / chemistry*
  • Drug Carriers / metabolism
  • Gene Transfer Techniques*
  • Humans
  • Intracellular Space / metabolism
  • Models, Molecular
  • Nucleic Acid Conformation
  • Oxidation-Reduction
  • Polymers / chemistry*
  • Polymers / metabolism
  • Reactive Oxygen Species / metabolism*

Substances

  • Drug Carriers
  • Polymers
  • Reactive Oxygen Species
  • DNA