Identification and molecular characterization of Panax notoginseng virus A, which may represent an undescribed novel species of the genus Totivirus, family Totiviridae

Arch Virol. 2016 Mar;161(3):731-4. doi: 10.1007/s00705-015-2716-4. Epub 2015 Dec 12.

Abstract

A double-stranded RNA (dsRNA), PN-S, isolated from Panax notoginseng was sequenced and characterized. dsRNA PN-S was determined to be 5003 base pairs (bp) in length and to have a genome organization similar to that of totiviruses. The two deduced proteins encoded by dsRNA PN-S have the highest amino acid (aa) sequence identity to CP and RNA-dependent RNA polymerase (RdRp) of black raspberry virus F (BrVF), a tentative member of the genus Totivirus, family Totiviridae, at 39% and 54%, respectively. In a phylogenetic tree based on an alignment of the RdRp amino acid sequences of dsRNA PN-S and selected totiviruses, dsRNA PN-S formed a monophyletic clade with dsRNA viruses belonging to the genus Totivirus. These data suggest that dsRNA PN-S may represent the genome of a member of a novel species of the genus Totivirus, family Totiviridae, for which we propose the name Panax notoginseng virus A (PnVA).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsid Proteins / genetics
  • Cluster Analysis
  • Gene Order
  • Genome, Viral*
  • Molecular Sequence Data
  • Open Reading Frames
  • Panax notoginseng / virology*
  • Phylogeny
  • RNA, Viral / genetics*
  • RNA-Dependent RNA Polymerase / genetics
  • Sequence Analysis, DNA*
  • Sequence Homology
  • Totiviridae / classification*
  • Totiviridae / genetics
  • Totiviridae / isolation & purification*

Substances

  • Capsid Proteins
  • RNA, Viral
  • RNA-Dependent RNA Polymerase

Associated data

  • GENBANK/KT388111