SNaPBceBcon: a Practical Tool for Identification and Genotyping of Burkholderia cepacia and Burkholderia contaminans

Ricardo Araujo; Rita Caramalho; Carla Coutinho; Isabel Sá-Correia

doi:10.1128/JCM.02476-15

SNaPBceBcon: a Practical Tool for Identification and Genotyping of Burkholderia cepacia and Burkholderia contaminans

J Clin Microbiol. 2016 Feb;54(2):483-8. doi: 10.1128/JCM.02476-15. Epub 2015 Dec 9.

Authors

Ricardo Araujo¹, Rita Caramalho², Carla Coutinho³, Isabel Sá-Correia³

Affiliations

¹ IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal ricjparaujo@yahoo.com.
² IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Innsbruck, Austria.
³ iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.

Abstract

We propose an optimized protocol for an extensive population analysis of Burkholderia cepacia and Burkholderia contaminans. Seven new polymorphisms were added to the recently proposed SNaPBcen assay, and a total of 18 markers ensured the clear identification and distinction of B. cepacia and B. contaminans isolates and high genotypic discrimination (Simpson index of 0.94) compared to those for multilocus sequence typing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Burkholderia Infections / diagnosis
Burkholderia Infections / etiology
Burkholderia Infections / microbiology
Burkholderia cepacia / classification*
Burkholderia cepacia / genetics*
Burkholderia cepacia complex / classification
Burkholderia cepacia complex / genetics
Cystic Fibrosis / complications
Genotype*
Genotyping Techniques*
Humans
Multilocus Sequence Typing
Polymorphism, Single Nucleotide

Grants and funding

IPATIMUP integrates the i3S Research Unit, which is partially supported by FCT, the Portuguese Foundation for Science and Technology. Funding received by iBB–Institute for Bioengineering and Biociences from FCT (UID/BIO/04565/2013) and from Programa Operacional Regional de Lisboa 2020 (project N.007317) is acknowledged. C.C. is the recipient of a postdoctoral fellowship grant from FCT (SFRH/BPD/81220/2011). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.