Abstract
When expressing plant cell wall degrading enzymes in the widely used tobacco (Nicotiana benthamiana) after Agrobacterium infiltration, difficulties arise due to the thin leaf structure. Thick leaved succulents, Kalanchoe blossfeldiana and Hylotelephium telephium, were tested as alternatives. A xyloglucanase, as well as a xyloglucanase inhibitor protein was successfully produced.
Keywords:
Agrobacterium; Crassulaceae; Xyloglucanase; Xyloglucanase inhibitor.
Published by Elsevier B.V.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Agrobacterium / enzymology
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Agrobacterium / genetics
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Agrobacterium / growth & development
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Agrobacterium / physiology*
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics
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Crassulaceae / metabolism
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Genes, Plant
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Glycoside Hydrolases / antagonists & inhibitors
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Glycoside Hydrolases / biosynthesis
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Glycoside Hydrolases / genetics
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Kalanchoe / microbiology*
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Nicotiana / microbiology
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Plant Leaves / microbiology*
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Plants, Genetically Modified
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Plasmids / genetics
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Sedum / microbiology
Substances
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Bacterial Proteins
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Glycoside Hydrolases
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xyloglucan endo(1-4)-beta-D-glucanase