Molecular characterization of Newcastle disease virus strains isolated from different outbreaks in Northeast India during 2014-15

Microb Pathog. 2016 Feb:91:85-91. doi: 10.1016/j.micpath.2015.11.026. Epub 2015 Nov 30.

Abstract

Newcastle disease virus (NDV) isolates recovered from different outbreaks in chicken flocks in Assam during 2014-15 were genotypically and pathotypically characterized. Nucleotide sequence analysis of fusion (F) and hemagglutinin protein genes showed a close similarity with genotype XIII strains of NDV. Amino acid sequence of F protein showed a virulent cleavage site (112)R-R-Q-K-R-F(117). Furthermore, pathogenicity test in one-day-old chicks and embryonated chicken eggs showed a virulent pathotype of the isolated NDV strains. The study will help us to understand the biology of circulating strains of NDV in Northeastern part of India.

Keywords: Newcastle disease virus; Outbreak; Pathogenicity; Virulent.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens
  • Disease Outbreaks
  • Genotype
  • India / epidemiology
  • Molecular Sequence Data
  • Newcastle Disease / epidemiology
  • Newcastle Disease / virology*
  • Newcastle disease virus / classification
  • Newcastle disease virus / genetics*
  • Newcastle disease virus / isolation & purification*
  • Phylogeny
  • Poultry Diseases / epidemiology
  • Poultry Diseases / virology*