Newcastle disease virus (NDV) isolates recovered from different outbreaks in chicken flocks in Assam during 2014-15 were genotypically and pathotypically characterized. Nucleotide sequence analysis of fusion (F) and hemagglutinin protein genes showed a close similarity with genotype XIII strains of NDV. Amino acid sequence of F protein showed a virulent cleavage site (112)R-R-Q-K-R-F(117). Furthermore, pathogenicity test in one-day-old chicks and embryonated chicken eggs showed a virulent pathotype of the isolated NDV strains. The study will help us to understand the biology of circulating strains of NDV in Northeastern part of India.
Keywords: Newcastle disease virus; Outbreak; Pathogenicity; Virulent.
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