Oleacein enhances anti-inflammatory activity of human macrophages by increasing CD163 receptor expression

Phytomedicine. 2015 Dec 15;22(14):1255-61. doi: 10.1016/j.phymed.2015.10.005. Epub 2015 Nov 1.

Abstract

Background: Oleacein (dialdehydic form of decarboxymethyl elenolic acid linked to hydroxytyrosol; 3,4-DHPEA-EDA) have been proven to possess antioxidant and anti-inflammatory activity.

Purpose: In this study, we examined whether oleacein could increase CD163 and IL-10 receptor expression as well as HO-1 intracellular secretion in human macrophages.

Methods: Effect of oleacein (10 and 20 μmol/l) or oleacein together with complexes of haemoglobin (Hb) and haptoglobin 1-1 (Hp11) or haptoglobin 2-2 (Hp22) on expression of IL-10 and CD163 receptor was determined by Flow Cytometry. Expression of CD163mRNA was measured by real-time quantitative RT-PCR. Heme oxygenase 1 (HO-1) intracellular secretion in macrophages was investigated by enzyme-linked immunosorbent assay (ELISA).

Results: Oleacein (OC) together with complexes HbHp11 or HbHp22 stimulated the expression of CD163 (30-100-fold), IL-10 (170-300-fold) and HO-1 secretion (60-130-fold) after 5 days of coincubation. The 2-fold (24 h), 4-fold (48 h) increase of CD163 mRNA level and its final (72 h) decrease was also observed.

Conclusion: Our results suggested that oleacein enhances anti-inflammatory activity of complexes haemoglobin with haptoglobin 1-1 and 2-2 and could play a potential role in the prevention of inflammatory disease related to atherosclerosis.

Keywords: Atherosclerosis; CD163; Carotid plaque; Macrophages; Oleacein.

MeSH terms

  • Aldehydes / pharmacology*
  • Anti-Inflammatory Agents / pharmacology*
  • Antigens, CD / metabolism*
  • Antigens, Differentiation, Myelomonocytic / metabolism*
  • Antioxidants / pharmacology
  • Cells, Cultured
  • Gene Expression / drug effects
  • Haptoglobins / pharmacology
  • Heme Oxygenase-1 / metabolism
  • Hemoglobins / pharmacology
  • Humans
  • Macrophages / drug effects*
  • Macrophages / metabolism
  • Phenols / pharmacology*
  • Pyrans
  • Receptors, Cell Surface / metabolism*
  • Receptors, Interleukin-10 / metabolism

Substances

  • 3,4-dihydroxyphenylethanol-elenolic acid dialdehyde
  • Aldehydes
  • Anti-Inflammatory Agents
  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • Antioxidants
  • CD163 antigen
  • Haptoglobins
  • Hemoglobins
  • Phenols
  • Pyrans
  • Receptors, Cell Surface
  • Receptors, Interleukin-10
  • oleacein
  • HMOX1 protein, human
  • Heme Oxygenase-1