Lilium floral fragrance: A biochemical and genetic resource for aroma and flavor

Timothy S Johnson; Michael L Schwieterman; Joo Young Kim; Keun H Cho; David G Clark; Thomas A Colquhoun

doi:10.1016/j.phytochem.2015.11.010

Lilium floral fragrance: A biochemical and genetic resource for aroma and flavor

Phytochemistry. 2016 Feb:122:103-112. doi: 10.1016/j.phytochem.2015.11.010. Epub 2015 Dec 1.

Authors

Timothy S Johnson¹, Michael L Schwieterman², Joo Young Kim³, Keun H Cho³, David G Clark², Thomas A Colquhoun⁴

Affiliations

¹ Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611, USA; Plant Innovation Center, University of Florida, Gainesville, FL 32611, USA.
² Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611, USA; Environmental Horticulture Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA; Plant Innovation Center, University of Florida, Gainesville, FL 32611, USA.
³ Environmental Horticulture Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA; Plant Innovation Center, University of Florida, Gainesville, FL 32611, USA.
⁴ Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611, USA; Environmental Horticulture Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA; Plant Innovation Center, University of Florida, Gainesville, FL 32611, USA. Electronic address: ucntcme1@ufl.edu.

PMID: 26654856
DOI: 10.1016/j.phytochem.2015.11.010

Abstract

Hybrid Lilium (common name lily) cultivars are among the top produced domestic fresh cut flowers and potted plants in the US today. Many hybrid Lilium cultivars produce large and showy flowers that emit copious amounts of volatile molecules, which can negatively affect a consumer's appreciation or limit use of the plant product. There are few publications focused on the biochemistry, genetics, and/or molecular regulation of floral volatile biosynthesis for Lilium cultivars. In an initial pursuit to provide breeders with molecular markers for floral volatile biosynthesis, a total of five commercially available oriental and oriental-trumpet hybrid Lilium cultivars were selected for analytical characterization of floral volatile emission. In total, 66 volatile molecules were qualified and quantitated among all cultivars. Chemical classes of identified volatiles include monoterpene hydrocarbons, monoterpene alcohols and aldehydes, phenylpropanoids, benzenoids, fatty-acid-derived, nitrogen-containing, and amino-acid-derived compounds. In general, the floral volatile profiles of the three oriental-trumpet hybrids were dominated by monoterpene hydrocarbons, monoterpene alcohols and aldehydes, while the two oriental hybrids were dominated by monoterpene alcohols and aldehydes and phenylpropanoids, respectively. Tepal tissues (two petal whirls) emitted the vast majority of total volatile molecules compared to the reproductive organs of the flowers. Tepal volatile profiles were cultivar specific with a high degree of distinction, which indicates the five cultivars chosen will provide an excellent differential genetic environment for gene discovery through comparative transcriptomics in the future. Cloning and assaying transcript accumulation from four floral volatile biosynthetic candidates provided few immediate or obvious trends with floral volatile emission.

Keywords: Floral volatile profiling and quantitation; GC–MS; Interspecific lily hybrids; Liliaceae; Lilium section Archelirion; Metabolic profiling; Oriental lily hybrids; Oriental-trumpet lily hybrids; Phenylpropanoids; Tepals; Terpenes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Flowers / genetics
Gene Expression Regulation, Plant
Lilium / chemistry*
Lilium / genetics
Odorants