Discrimination of reactively-dyed cotton fibres with thin layer chromatography and UV microspectrophotometry

K De Wael; K Van Dijck; F Gason

doi:10.1016/j.scijus.2015.06.001

Discrimination of reactively-dyed cotton fibres with thin layer chromatography and UV microspectrophotometry

Sci Justice. 2015 Dec;55(6):422-30. doi: 10.1016/j.scijus.2015.06.001. Epub 2015 Jun 26.

Authors

K De Wael¹, K Van Dijck², F Gason²

Affiliations

¹ Nationaal Instituut voor Criminalistiek en Criminologie, Vilvoordsesteenweg 100, B-1120 Brussel, Belgium. Electronic address: kris.dewael@just.fgov.be.
² Nationaal Instituut voor Criminalistiek en Criminologie, Vilvoordsesteenweg 100, B-1120 Brussel, Belgium. Electronic address: http://www.nicc.fgov.be.

PMID: 26654077
DOI: 10.1016/j.scijus.2015.06.001

Abstract

Reactively-dyed black, navy blue and medium red cotton samples showing metamerism under fluorescent tube illumination were examined. Optical microscopy (bright field, polarization and fluorescence microscopy) was used, followed by microspectrometry in the visible range (MSP Vis), to differentiate the samples in each block of colours. Additionally, the non-discriminated samples were subjected both to microspectrophotometry in the UV-range (MSP UV) and to enzymatic digestion followed by high performance thin layer chromatography (HPTLC) on the digests. While it was found that both methods may potentially result in higher discrimination, preparation of reactively-dyed cotton for HPTLC was found to be a very tedious and time-consuming step and HPTLC only led to a better discrimination than MSP UV for the red cotton samples. The results suggest that in order to increase the discrimination for reactively-dyed cotton fibres, measurement of the UV absorption spectrum (MSP UV) is preferred over HPTLC.

Keywords: Discriminating power; Enzymatic digestion; Forensic fibre examination; Microspectrophotometry; Optical microscopy; Thin layer chromatography.