Abstract
The actin (ACT) gene from the budding yeast Kluyveromyces lactis was cloned, and the nucleotide sequence was determined. The gene had a single intron 778 nucleotides in length which possessed the highly conserved splicing signals found in Saccharomyces cerevisiae introns. We demonstrated splicing of heterologous ACT transcripts in both K. lactis and S. cerevisiae.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Actins / genetics
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Amino Acid Sequence
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Base Sequence
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DNA, Fungal / genetics
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Genes, Fungal*
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Introns
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Kluyveromyces / genetics*
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Kluyveromyces / metabolism
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Molecular Sequence Data
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RNA Precursors / genetics
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RNA Precursors / metabolism
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RNA Splicing*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Saccharomycetales / genetics*
Substances
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Actins
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DNA, Fungal
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RNA Precursors