Targeting Innate Immunity with dsRNA-Conjugated Mesoporous Silica Nanoparticles Promotes Antitumor Effects on Breast Cancer Cells

Chemistry. 2016 Jan 26;22(5):1582-6. doi: 10.1002/chem.201504629. Epub 2016 Jan 7.

Abstract

We describe herein a Toll-like receptor 3 (TLR3) targeting delivery system based on mesoporous silica nanoparticles capped with the synthetic double stranded RNA polyinosinic-polycytidylic acid (poly(I:C)) for controlled cargo delivery in SK-BR-3 breast carcinoma cells. Our results show that poly(I:C)-conjugated nanoparticles efficiently targeted breast cancer cells due to dsRNA-TLR3 interaction. Such interaction also triggered apoptotic pathways in SK-BR-3, significantly decreasing cells viability. Poly(I:C) cytotoxic effect in breast carcinoma cells was enhanced by loading nanoparticles' mesopores with the anthracyclinic antibiotic doxorubicin, a commonly used chemotherapeutic agent.

Keywords: antitumor agents; breast carcinoma; drug delivery; mesoporous gated materials; polyinosinic-polycytidylic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemistry*
  • Antineoplastic Agents / pharmacology*
  • Breast Neoplasms / chemistry*
  • Breast Neoplasms / drug therapy*
  • Cell Line, Tumor
  • Doxorubicin / chemistry*
  • Female
  • Humans
  • Immunity, Innate
  • Nanoparticles / chemistry*
  • Poly I-C / chemistry*
  • Poly I-C / pharmacology*
  • RNA, Double-Stranded / chemistry*
  • RNA, Double-Stranded / pharmacology
  • Silicon Dioxide / chemistry*

Substances

  • Antineoplastic Agents
  • RNA, Double-Stranded
  • Silicon Dioxide
  • Doxorubicin
  • Poly I-C