Installation of a Rigid EDTA-Like Motif into a Protein α-Helix for Paramagnetic NMR Spectroscopy with Cobalt(II) Ions

James D Swarbrick; Phuc Ung; Matthew L Dennis; Michael D Lee; Sandeep Chhabra; Bim Graham

doi:10.1002/chem.201503139

Installation of a Rigid EDTA-Like Motif into a Protein α-Helix for Paramagnetic NMR Spectroscopy with Cobalt(II) Ions

Chemistry. 2016 Jan 22;22(4):1228-32. doi: 10.1002/chem.201503139. Epub 2015 Dec 17.

Authors

James D Swarbrick¹, Phuc Ung², Matthew L Dennis², Michael D Lee², Sandeep Chhabra², Bim Graham³

Affiliations

¹ Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, 3052, Victoria, Australia. james.swarbrick@monash.edu.
² Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, 3052, Victoria, Australia.
³ Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, 3052, Victoria, Australia. bim.graham@monash.edu.

PMID: 26634335
DOI: 10.1002/chem.201503139

Abstract

Coupling two copies of an iminodiacetic acid-cysteine hybrid ligand to a pair of cysteine residues positioned in an i, i+4 arrangement within a protein α-helix leads to generation of an EDTA-like metal ion-binding motif. Rigid binding of a Co(II) ion by this motif produces pseudo-contact shifts suitable for paramagnetic NMR structural studies.

Keywords: alpha-helix; cobalt; paramagnetic NMR spectroscopy; protein structures; pseudo-contact shifts.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Binding Sites
Cobalt / chemistry*
Edetic Acid / chemistry*
Ligands
Magnetic Resonance Spectroscopy
Protein Binding

Substances

Ligands
Cobalt
Edetic Acid