The parallel reaction monitoring (PRM) assay has emerged as an alternative method of targeted quantification. The PRM assay is performed in a high resolution and high mass accuracy mode on a mass spectrometer. This review presents the features that make PRM a highly specific and selective method for targeted quantification using quadrupole-Orbitrap hybrid instruments. In addition, this review discusses the label-based and label-free methods of quantification that can be performed with the targeted approach.
Keywords: PRM; Q-Exactive; SRM; accurate mass; high resolution; mass spectrometry; parallel reaction monitoring; proteomics; quantitative proteomics; selected reaction monitoring; targeted.