Enzymatic monitoring of lignin and lignin derivatives biooxidation

Victor Ibrahim; Gashaw Mamo

doi:10.1016/j.mimet.2015.11.015

Enzymatic monitoring of lignin and lignin derivatives biooxidation

J Microbiol Methods. 2016 Jan:120:53-5. doi: 10.1016/j.mimet.2015.11.015. Epub 2015 Nov 26.

Authors

Victor Ibrahim¹, Gashaw Mamo²

Affiliations

¹ Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
² Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden. Electronic address: gashaw.mamo@biotek.lu.se.

PMID: 26632344
DOI: 10.1016/j.mimet.2015.11.015

Abstract

Lignin oxidation was enzymatically monitored by measuring methanol released during the reaction. The methanol was oxidized to formaldehyde and hydrogen peroxide, and the latter used to oxidize ABTS to a product measured spectrophotometrically. The efficiency was comparable to the commonly used gas chromatography method. The assay was fast and inexpensive.

Keywords: Gas chromatography; Horse radish peroxidase; Laccase; Lignin; Methanol determination.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques / methods*
Chromatography, Gas / methods
Enzyme Assays / methods*
Formaldehyde / analysis
Formaldehyde / chemistry
Horseradish Peroxidase / metabolism
Hydrogen Peroxide / chemistry
Laccase / analysis
Laccase / chemistry
Lignin / chemistry
Lignin / metabolism*
Methanol / analysis
Methanol / chemistry
Oxidation-Reduction*
Oxidoreductases / chemistry
Oxidoreductases / metabolism
Spectrophotometry / methods

Substances

Formaldehyde
Lignin
Hydrogen Peroxide
Oxidoreductases
Laccase
Horseradish Peroxidase
Methanol