This study investigated laterality of manual pushing during a gait cycle by measuring pushing force, muscular activation and foot pressure. Subjects were 17 healthy young adult males; (11 right-handed [RH], and 6 left-handed [LH]). They pushed a force plate while walking on a treadmill at 1.5, 3, and 4 km/h. Electromyogram (EMG) data were collected bilaterally from the tibialis anterior, soleus, lumbar erector spinae and triceps brachii. To measure foot pressure, ten pressure sensors were attached bilaterally on five points of the sole. Symmetry assessment was performed by comparing bilateral data and cross-correlation function (CCF). Gait cycle duration was found to be symmetrical in all conditions. LH subjects demonstrated asymmetry in calcaneus contact duration to control ankle flexion, whereas RH were symmetrical. Velocity affected tibialis anterior muscle time lag and soleus muscle CCF coefficients, mainly in LH. We found that triceps brachii muscle CCF coefficients in LH subjects were affected by increasing velocity. Results indicated that LH and RH did not mirror each other, since both had distinct characteristics. Furthermore these asymmetries were not strictly associated with the preferred side, indicating that generalisation of preferred side to whole-body coordination should be avoided, since we could not separate one side from the other.