Supportless electrochemical sensor based on molecularly imprinted polymer modified nanoporous microrod for determination of dopamine at trace level

Yingchun Li; Han Song; Lu Zhang; Peng Zuo; Bang-Ce Ye; Jun Yao; Wen Chen

doi:10.1016/j.bios.2015.11.063

Supportless electrochemical sensor based on molecularly imprinted polymer modified nanoporous microrod for determination of dopamine at trace level

Biosens Bioelectron. 2016 Apr 15:78:308-314. doi: 10.1016/j.bios.2015.11.063. Epub 2015 Nov 23.

Authors

Yingchun Li¹, Han Song², Lu Zhang², Peng Zuo³, Bang-Ce Ye⁴, Jun Yao⁵, Wen Chen⁶

Affiliations

¹ Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, PR China; Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China. Electronic address: yingchunli@shzu.edu.cn.
² Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, PR China.
³ Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
⁴ Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China; Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
⁵ School of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang 830011, PR China. Electronic address: xydyaojun@163.com.
⁶ Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, PR China. Electronic address: cw_pha@shzu.edu.cn.

PMID: 26630285
DOI: 10.1016/j.bios.2015.11.063

Abstract

In this work, we developed a novel freestanding metallic microrod as working electrode for highly sensitive and selective electrochemical detection of trace dopamine (DA). The electrode was facilely fabricated via first dealloying smooth Au-Ag alloy microrod (AMR) into nanoporous Au-Ag alloy microrod (NPAMR) and further modifying with electro-polymerized molecularly imprinted polymer (MIP). Influencing factors during electro-polymerization process including pH value and molar ratio of monomer to template molecule were optimized. Under the optimal conditions, a linear range from 2 × 10(-13) to 2 × 10(-8)M for measuring DA was obtained with an ultralow detection limit of 7.63 × 10(-14)M (S/N=3). In addition, the MIP-modified electrode (MIP/NPAMR) was successfully employed to test DA in serum and brain samples.

Keywords: Dopamine; Electrochemical sensor; Molecularly imprinted polymer; Nanoporous alloy microrod; Trace measurement.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques*
Dopamine / chemistry
Dopamine / isolation & purification*
Electrochemical Techniques*
Gold / chemistry
Humans
Limit of Detection
Polymers / chemistry

Substances

Polymers
Gold
Dopamine