Generation of Chimeras from Porcine Induced Pluripotent Stem Cells

Methods Mol Biol. 2015:1330:153-67. doi: 10.1007/978-1-4939-2848-4_14.

Abstract

Pig induced pluripotent stem cells (piPSCs) offer a great opportunity and a number of advantages in the generation of transgenic animals. These immortalized cells can undergo multiple rounds of genetic modifications (e.g., gene knock-in, knockout) and selection leading to animals that have optimized traits of biomedical or agricultural interests. In this chapter we describe the production and characterization of piPSCs, microinjection of piPSCs into embryos, embryo transfer and production of chimeric animals based on successful protocols.

Keywords: Embryo microinjection; Embryo transfer; Induced pluripotent stem cells; Pig.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Cellular Reprogramming*
  • Embryo Transfer
  • Embryo, Mammalian / cytology
  • Female
  • Genetic Vectors / genetics
  • Immunohistochemistry
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism*
  • Microinjections
  • Pregnancy
  • Swine
  • Transcription Factors / genetics
  • Transduction, Genetic

Substances

  • Transcription Factors