Antileishmanial diterpenoid alkaloids from Aconitum spicatum (Bruhl) Stapf

Sajan L Shyaula; Tensangmu Tamang; Nida Ghouri; Achyut Adhikari; Shanti Marasini; Gan B Bajracharya; Mangala D Manandhar; Muhammad Iqbal Choudhary

doi:10.1080/14786419.2015.1114941

Antileishmanial diterpenoid alkaloids from Aconitum spicatum (Bruhl) Stapf

Nat Prod Res. 2016 Nov;30(22):2590-2593. doi: 10.1080/14786419.2015.1114941. Epub 2015 Nov 30.

Authors

Sajan L Shyaula¹, Tensangmu Tamang², Nida Ghouri³, Achyut Adhikari³, Shanti Marasini², Gan B Bajracharya¹, Mangala D Manandhar¹, Muhammad Iqbal Choudhary^{3

4}

Affiliations

¹ a Faculty of Science , Nepal Academy of Science and Technology , Lalitpur , Nepal.
² b Department of Chemistry, Tri-Chandra Multiple Campus , Tribhuvan University , Kathmandu , Nepal.
³ c HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences , University of Karachi , Karachi , Pakistan.
⁴ d Faculty of Science, Department of Biochemistry , King Abdulaziz University , Jeddah , Saudi Arabia.

PMID: 26615865
DOI: 10.1080/14786419.2015.1114941

Abstract

The crude extracts of tubers of Aconitum spicatum (Bruhl) Stapf were investigated for in vitro antileishmanial activity against Leishmania major. The dichloromethane extract at pH 2.5 showed antileishmanial activity with IC₅₀ value of 27.10 ± 0.0 μg/mL. Chromatographic purification of the dichloromethane extract led to isolation of three C-19 norditerpenoid alkaloids indaconitine (1), chasmaconitine (2) and ludaconitine (3). Compounds 3 and 2 showed antileishmanial activity with IC₅₀ = 36.10 ± 3.4 and 56.30 ± 2.1 μg/mL, respectively. Compound 1 was less effective (IC₅₀ > 100 μg/mL). The cytotoxicity of compounds 1, 2 and 3 studied against MCF7, HeLa and PC3 cancer cell lines and 3T3 normal fibroblast cell line did not show cytotoxicity at 30 μM.

Keywords: Aconitum spicatum; Leishmania major; antileishmanial activity; diterpenoid alkaloids.