Quantifying the 'escapers' among RNA species

Iolanda Ferro; Zoya Ignatova

doi:10.1042/BST20150158

Quantifying the 'escapers' among RNA species

Biochem Soc Trans. 2015 Dec;43(6):1215-20. doi: 10.1042/BST20150158.

Authors

Iolanda Ferro¹, Zoya Ignatova²

Affiliations

¹ Biochemistry and Molecular Biology, Dept. of Chemistry and Biochemistry, University of Hamburg, Hamburg 20146, Germany.
² Biochemistry and Molecular Biology, Dept. of Chemistry and Biochemistry, University of Hamburg, Hamburg 20146, Germany zoya.ignatova@chemie.uni-hamburg.de.

PMID: 26614663
DOI: 10.1042/BST20150158

Abstract

tRNAs are fundamental components of translation and emerging evidence places them more centrally in various other cellular processes. However, rather than being uniformly conserved, tRNA abundance is instead highly variable and adaptable. The amount of tRNA genes greatly differs among species. Moreover, even within the same genome, tRNA abundance shapes the proteome in a tissue- and cell-specific manner and is dynamically regulated in response to stress. Here, we review approaches for identification and quantification of tRNAs and their functional integrity. We discuss the resolution of each method and highlight new approaches with cell-wide resolution based on deep-sequencing technologies.

Keywords: ribonucleic acid; transfer ribonucleic acid (tRNA); transfer ribonucleic acid (tRNA) modification; transfer ribonucleic acid (tRNA)-sequencing; translation.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Anticodon / genetics
Base Sequence
Chromatography, High Pressure Liquid / methods
Electrophoresis, Gel, Two-Dimensional / methods
High-Throughput Nucleotide Sequencing / methods*
Nucleic Acid Conformation
Oligonucleotide Array Sequence Analysis / methods*
RNA / chemistry
RNA / genetics*
RNA / metabolism
RNA, Transfer / chemistry
RNA, Transfer / genetics*
RNA, Transfer / metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods

Substances

Anticodon
RNA
RNA, Transfer