MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e

Zhicheng Liu; Yuru Zhou; Yue Yuan; Fang Nie; Rui Peng; Qianyin Li; Zhongshi Lyu; Zhaomin Mao; Liyuan Huang; Li Zhou; Yiman Li; Jing Hao; Dongsheng Ni; Qianni Jin; Yaoshui Long; Pan Ju; Wen Yu; Jianing Liu; Yanxia Hu; Qin Zhou

doi:10.3390/ijms161126075

MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e

Int J Mol Sci. 2015 Nov 24;16(11):27945-55. doi: 10.3390/ijms161126075.

Authors

Zhicheng Liu¹, Yuru Zhou², Yue Yuan³, Fang Nie⁴, Rui Peng⁵, Qianyin Li⁶, Zhongshi Lyu⁷, Zhaomin Mao⁸, Liyuan Huang⁹, Li Zhou¹⁰, Yiman Li¹¹, Jing Hao¹², Dongsheng Ni¹³, Qianni Jin¹⁴, Yaoshui Long¹⁵, Pan Ju¹⁶, Wen Yu¹⁷, Jianing Liu¹⁸, Yanxia Hu¹⁹, Qin Zhou²⁰

Affiliations

¹ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. liuzhicheng323@163.com.
² The Seventh Class of 2012 year entry, the Third Clinical College, Chongqing Medical University, Chongqing 400016, China. zhouyuru93@163.com.
³ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. yyokyy1126@hotmail.com.
⁴ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. nifang168@163.com.
⁵ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. pengrui911@foxmail.com.
⁶ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lqianyincqmu@163.com.
⁷ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. Zhongshilyu@163.com.
⁸ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. mao1204086118@163.com.
⁹ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lyhuang0603@sina.com.
¹⁰ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. shmily900519@foxmail.com.
¹¹ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. 18715854036@sina.cn.
¹² The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lanyxiu@163.com.
¹³ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. cqmunds@163.com.
¹⁴ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. qiannijincqmu@sina.com.
¹⁵ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lys960110@sina.com.
¹⁶ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. 18883936591@163.com.
¹⁷ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. yuwen0717@sina.com.
¹⁸ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. keithljn@sina.cn.
¹⁹ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. hyx_zuichu@outlook.com.
²⁰ The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. zhouqin@cqmu.edu.cn.

Abstract

Accumulating evidence demonstrated that miRNAs are highly involved in kidney fibrosis and Epithelial-Eesenchymal Transition (EMT), however, the mechanisms of miRNAs in kidney fibrosis are poorly understood. In this work, we identified that miR542-3p could promote EMT through down-regulating bone morphogenetic protein 7 (BMP7) expression by targeting BMP7 3'UTR. Firstly, real-time PCR results showed that miR542-3p was significantly up-regulated in kidney fibrosis in vitro and in vivo. Moreover, Western blot results demonstrated that miR542-3p may promote EMT in the NRK52e cell line. In addition, we confirmed that BMP7, which played a crucial role in anti-kidney fibrosis and suppressed the progression of EMT, was a target of miR542-3p through Dual-Luciferase reporter assay, as did Western blot analysis. The effects of miR542-3p on regulating EMT could also be suppressed by transiently overexpressing BMP7 in NRK52e cells. Taken together, miR542-3p may be a critical mediator of the induction of EMT via directly targeting BMP7.

Keywords: Epithelial-Eesenchymal Transition (EMT); bone morphogenetic protein 7 (BMP7); kidney fibrosis; miR542-3p; transforming growth factor beta 1 (TGFβ1).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Binding Sites
Bone Morphogenetic Protein 7 / chemistry
Bone Morphogenetic Protein 7 / genetics*
Cell Line
Disease Models, Animal
Epithelial-Mesenchymal Transition / genetics*
Fibrosis
Gene Expression Regulation
Humans
Kidney Diseases / genetics
Kidney Diseases / pathology
Mice
MicroRNAs / chemistry
MicroRNAs / genetics*
RNA Interference*
RNA, Messenger / chemistry
RNA, Messenger / genetics
Transforming Growth Factor beta1 / metabolism

Substances

Bone Morphogenetic Protein 7
MIRN542 microRNA, human
MicroRNAs
RNA, Messenger
Transforming Growth Factor beta1