A bacterial homologue of the human iron exporter ferroportin

FEBS Lett. 2015 Dec 21;589(24 Pt B):3829-35. doi: 10.1016/j.febslet.2015.11.025. Epub 2015 Nov 19.

Abstract

A bacterial homologue of the human iron exporter ferroportin found in the predatory Gram-negative bacterium Bdellovibrio bacteriovorus has been investigated. Molecular modelling, expression in recombinant form and iron binding and transport assays demonstrate that B. bacteriovorus ferroportin (bdFpn) is indeed an orthologue of human ferroportin. Key residues corresponding to those essential for iron binding and transport in human ferroportin are conserved in the bacterial homologue and are predicted to be correctly clustered in the central cavity of the protein. Mutation of these residues grossly affects the iron binding and transport ability of bdFpn.

Keywords: Bdellovibrio bacteriovorus; Ferroportin; Iron; Major facilitator superfamily.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Bdellovibrio*
  • Cation Transport Proteins / chemistry*
  • Cation Transport Proteins / genetics
  • Cation Transport Proteins / metabolism*
  • Conserved Sequence
  • Humans
  • Iron / metabolism*
  • Models, Molecular
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Mutation
  • Protein Conformation
  • Protein Transport
  • Sequence Homology, Amino Acid*

Substances

  • Bacterial Proteins
  • Cation Transport Proteins
  • metal transporting protein 1
  • Iron