Interactions of two cytotoxic organoruthenium(II) complexes with G-quadruplex

Sara Seršen; Primož Šket; Janez Plavec; Iztok Turel

doi:10.1016/j.jinorgbio.2015.11.012

Interactions of two cytotoxic organoruthenium(II) complexes with G-quadruplex

J Inorg Biochem. 2016 Jul:160:70-7. doi: 10.1016/j.jinorgbio.2015.11.012. Epub 2015 Nov 11.

Authors

Sara Seršen¹, Primož Šket², Janez Plavec³, Iztok Turel⁴

Affiliations

¹ Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, SI-1000 Ljubljana, Slovenia.
² EN-FIST Center of Excellence, SI-1000 Ljubljana, Slovenia; Slovenian NMR Center, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia.
³ Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, SI-1000 Ljubljana, Slovenia; EN-FIST Center of Excellence, SI-1000 Ljubljana, Slovenia; Slovenian NMR Center, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia.
⁴ Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, SI-1000 Ljubljana, Slovenia; EN-FIST Center of Excellence, SI-1000 Ljubljana, Slovenia. Electronic address: iztok.turel@fkkt.uni-lj.si.

PMID: 26606993
DOI: 10.1016/j.jinorgbio.2015.11.012

Abstract

Two previously isolated cytotoxic complexes [(η(6)-p-cymene)Ru(κ(2)-CF3COCHCOC5H3O)L](n+) (L=Cl (1); n=0, pta (2) (pta=1,3,5-triaza-7-phosphaadamantane); n=1) were investigated for their selectivity and ability to interact with DNA G-quadruplex adopted by d[G3ATG3ACACAG4ACG3] (3) whose topology exhibits diagonal, edge-type and double-chain reversal loops. Structural changes were followed using high-resolution NMR techniques in the presence of 1 and 2. Results showed weak interaction between the organoruthenium complexes and G-quadruplex. Moreover, no significant changes in thermal stability were confirmed by a UV-melting assay for both 1 and 2. These findings emphasize that anticancer activity of Ru(II) complexes may not be correlated with binding to nucleic acid like G-quadruplex.

Keywords: Anticancer activity; G-quadruplex; NMR study; Organoruthenium complex; UV melting.

MeSH terms

Adamantane / analogs & derivatives*
Adamantane / chemistry
Antineoplastic Agents / chemistry*
Binding Sites
Coordination Complexes / chemistry*
Crystallography, X-Ray
Cymenes
Deuterium
G-Quadruplexes*
Magnetic Resonance Spectroscopy
Molecular Conformation
Monoterpenes / chemistry*
Nucleic Acid Denaturation
Organometallic Compounds / chemistry*
Organophosphorus Compounds / chemistry*
Ruthenium / chemistry*
Ultraviolet Rays

Substances

1,3,5-triaza-7-phosphaadamantane
Antineoplastic Agents
Coordination Complexes
Cymenes
Monoterpenes
Organometallic Compounds
Organophosphorus Compounds
4-cymene
Ruthenium
Deuterium
Adamantane