Abstract
A novel bioink and a dispensing technique for 3D tissue-engineering applications are presented. The technique incorporates a coaxial extrusion needle using a low-viscosity cell-laden bioink to produce highly defined 3D biostructures. The extrusion system is then coupled to a microfluidic device to control the bioink arrangement deposition, demonstrating the versatility of the bioprinting technique. This low-viscosity cell-responsive bioink promotes cell migration and alignment within each fiber organizing the encapsulated cells.
Keywords:
3D tissue engineering; bioinks; bioprinting; microfluidics; vascular tissue engineering.
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Alginates / chemistry
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Bioprinting / instrumentation
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Bioprinting / methods*
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Cell Survival / radiation effects
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Extracellular Matrix / chemistry
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Extracellular Matrix / metabolism
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Gelatin / chemistry
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Glucuronic Acid / chemistry
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Hexuronic Acids / chemistry
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Human Umbilical Vein Endothelial Cells
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Humans
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Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry
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Microfluidic Analytical Techniques / instrumentation
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Microfluidic Analytical Techniques / methods*
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Microscopy, Confocal
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Printing, Three-Dimensional
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Tissue Engineering
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Tissue Scaffolds*
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Ultraviolet Rays
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Viscosity
Substances
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Alginates
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Hexuronic Acids
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Hydrogel, Polyethylene Glycol Dimethacrylate
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Glucuronic Acid
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Gelatin