Abstract
We describe a new phenotypic test to detect beta-lactamases. This assay is based on diffusion of beta-lactam/beta-lactamase through a bacterial filter. Beta-lactam hydrolysis on (the other side of) the filter leads to a change in antibiotic susceptibility, which can be measured by disc diffusion tests. We illustrate its ease of use to detect beta-lactamases of different classes.
Keywords:
Beta-lactamase; Carbapenemase; ESBL; Phenotypic method; Resistance.
Copyright © 2015 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacteria / drug effects
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Bacteria / enzymology*
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Bacteria / metabolism
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Bacterial Proteins / analysis
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Bacterial Proteins / chemistry*
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Bacteriological Techniques*
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Disk Diffusion Antimicrobial Tests
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Microbial Sensitivity Tests / methods
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Phenotype
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beta-Lactam Resistance
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beta-Lactamases / analysis
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beta-Lactamases / chemistry*
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beta-Lactams / metabolism
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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beta-Lactams
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beta-Lactamases
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carbapenemase