A wash step at collection of placental biopsies from preeclamptic pregnancies does not adversely affect levels of sFlt-1 or endoglin

Pregnancy Hypertens. 2015 Oct;5(4):294-7. doi: 10.1016/j.preghy.2015.06.003. Epub 2015 Jun 22.

Abstract

There is recent interest for uniform placental collection protocols so laboratories can share samples. However, concerns have been raised a wash step at collection causes significant loss of sFlt-1 and soluble endoglin, among the most studied proteins in placentology. We measured Flt-1 and endoglin mRNA and protein in 10 preeclamptic placentas that were washed, or left unwashed. Reassuringly, there was no significant change in the Flt-1, sFlt-1-e15a or sFlt-1-i13 mRNA or Flt-1 or sFlt-1 protein expression or localization. There was also no change in endoglin mRNA expression or protein localization. Washing preeclamptic placental samples does not alter anti-angiogenic factor expression.

Keywords: Preeclampsia; Soluble endoglin; sFlt1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antigens, CD / biosynthesis*
  • Biomarkers / metabolism
  • Biopsy
  • Body Mass Index
  • Case-Control Studies
  • Endoglin
  • Female
  • Humans
  • Placenta / metabolism*
  • Pre-Eclampsia / diagnosis
  • Pre-Eclampsia / metabolism*
  • Predictive Value of Tests
  • Pregnancy
  • Receptors, Cell Surface / biosynthesis*
  • Sensitivity and Specificity
  • Vascular Endothelial Growth Factor Receptor-1 / biosynthesis*

Substances

  • Antigens, CD
  • Biomarkers
  • ENG protein, human
  • Endoglin
  • Receptors, Cell Surface
  • FLT1 protein, human
  • Vascular Endothelial Growth Factor Receptor-1