Analysis of 30 insertion-deletion polymorphisms in the Japanese population using the Investigator DIPplex® kit

Miya Nunotani; Tetsuya Shiozaki; Noriko Sato; Sayako Kamei; Kanae Takatsu; Tokutaro Hayashi; Masao Ota; Hideki Asamura

doi:10.1016/j.legalmed.2015.10.002

Analysis of 30 insertion-deletion polymorphisms in the Japanese population using the Investigator DIPplex® kit

Leg Med (Tokyo). 2015 Nov;17(6):467-70. doi: 10.1016/j.legalmed.2015.10.002. Epub 2015 Oct 8.

Authors

Miya Nunotani¹, Tetsuya Shiozaki¹, Noriko Sato¹, Sayako Kamei¹, Kanae Takatsu¹, Tokutaro Hayashi¹, Masao Ota¹, Hideki Asamura²

Affiliations

¹ Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
² Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan. Electronic address: asamura@shinshu-u.ac.jp.

PMID: 26593991
DOI: 10.1016/j.legalmed.2015.10.002

Abstract

Allele frequencies and forensic parameters for 30 insertion-deletion polymorphisms (INDELs) were investigated in a sample of 251 unrelated Japanese individuals using the Investigator DIPplex® kit (QIAGEN). The frequency distributions showed no deviations from Hardy-Weinberg equilibrium expectations. The combined powers of discrimination and match probability for the 30 INDELs were 0.9999999998 and 2.67×10(-11), respectively. To assess the effectiveness of the kit in typing degraded DNA, an ancient bone sample of a Jomon skeleton was analyzed; most of 30 INDELs and amelogenin were typed successfully. We concluded that the kit offers considerable potential for personal identification from degraded DNA samples due to the small amplicon length and high degree of polymorphisms.

Keywords: Degraded DNA; Insertion–deletion polymorphisms (INDELs); Investigator DIPplex® kit; Japanese population.

MeSH terms

Female
Gene Deletion*
Genetics, Population / instrumentation*
Humans
Japan
Male
Polymorphism, Genetic*