Quantification of aroma constituents of mango sap from different Pakistan mango cultivars using gas chromatography triple quadrupole mass spectrometry

Abstract

In this study, a quantitative method was developed based on gas chromatography triple quadrupole mass spectrometry (GC-QQQ-MS) for the analysis of aroma component of mango sap (latex) in nine Pakistani varieties that are Anmol, Began pali, Badami, Caroba, Chaunsa, Lal patra, Neelum, Sohnara and Tota pari. The non-aqueous phase of sap was studied and a total seven selected terpenes that are α-pinene, α-phellandrene, (+)-3-carene, sabinene, γ-terpinene, (-)-trans-caryophyllene and α-humulene were quantified using GC-QQQ-MS in MRM (multiple reaction mode) mode. Calibration curves were generated and R(2)>0.99 was found for all analytes. Intra-day and inter-day precision (% R.S.D.) of the developed method was less than 4% while % accuracy was in the range of 95.3-105.1 for all analytes. Among all the varieties, Neelum contains the highest amount of four terpenes out of seven quantified terpenes.

Keywords: Aroma volatile compounds; GC–QQQ-MS; Non-aqueous phase of sap; Quantification.