In vitro evaluation of anti-diabetic activity and cytotoxicity of chemically analysed Ocimum basilicum extracts

Sleman Kadan; Bashar Saad; Yoel Sasson; Hilal Zaid

doi:10.1016/j.foodchem.2015.10.044

In vitro evaluation of anti-diabetic activity and cytotoxicity of chemically analysed Ocimum basilicum extracts

Food Chem. 2016 Apr 1:196:1066-74. doi: 10.1016/j.foodchem.2015.10.044. Epub 2015 Oct 22.

Authors

Sleman Kadan¹, Bashar Saad², Yoel Sasson³, Hilal Zaid⁴

Affiliations

¹ Qasemi Research Center - Al-Qasemi Academy, P.O. Box 124, Baqa El-Gharbia 30100, Israel; Casali Center for Applied Chemistry, Institute of Chemistry, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
² Qasemi Research Center - Al-Qasemi Academy, P.O. Box 124, Baqa El-Gharbia 30100, Israel; Faculty of Arts and Sciences, Arab American University Jenin, P.O. Box 240, Jenin, Palestine. Electronic address: bashar@qsm.ac.il.
³ Casali Center for Applied Chemistry, Institute of Chemistry, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
⁴ Qasemi Research Center - Al-Qasemi Academy, P.O. Box 124, Baqa El-Gharbia 30100, Israel; Faculty of Arts and Sciences, Arab American University Jenin, P.O. Box 240, Jenin, Palestine. Electronic address: hilalz@qsm.ac.il.

PMID: 26593590
DOI: 10.1016/j.foodchem.2015.10.044

Abstract

The aim of this study was to evaluate the role of glucose transporter-4 (GLUT4) in the anti-diabetic effects of methanol, hexane and dichloromethane extracts of the aerial parts of Ocimum basilicum (OB) and to analyze their phytochemical composition. Phytochemical analysis of the three extracts by GC/MS using the silylation derivatization technique revealed 53 compounds, 17 of them were found for the first time in OB. Cytotoxic and anti-diabetic properties of the extracts were evaluated using L6-GLUT4myc muscle cells stably expressing myc epitope at the exofacial loop (GLUT4). No cytotoxic effects were observed in treated cells up to 0.25 mg/ml extract as measured with MTT and LDH-leakage assays. GLUT4 translocation to the plasma membrane was elevated by 3.5 and 7 folds (-/+ insulin) after treatment with OB extracts for 20 h. Our findings suggest that the observed anti-diabetic properties of OB extracts are possibly mediated in part through one or more of the 17 new identified compound.

Keywords: Chemical composition; GC/MS; GLUT4; Ocimum basilicum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Diabetes Mellitus / drug therapy*
Glucose Transporter Type 4
Humans
In Vitro Techniques
Insulin / metabolism*
Models, Molecular
Ocimum basilicum / chemistry*
Plant Extracts / chemistry*

Substances

Glucose Transporter Type 4
Insulin
Plant Extracts
SLC2A4 protein, human