Applications of high dimensionality experiments to biomolecular NMR

Michał Nowakowski; Saurabh Saxena; Jan Stanek; Szymon Żerko; Wiktor Koźmiński

doi:10.1016/j.pnmrs.2015.07.001

Applications of high dimensionality experiments to biomolecular NMR

Prog Nucl Magn Reson Spectrosc. 2015 Nov:90-91:49-73. doi: 10.1016/j.pnmrs.2015.07.001. Epub 2015 Jul 11.

Authors

Michał Nowakowski¹, Saurabh Saxena¹, Jan Stanek¹, Szymon Żerko¹, Wiktor Koźmiński²

Affiliations

¹ Biological and Chemical Research Centre, Faculty of Chemistry, University of Warsaw, Żwirki i Wigury 101, 02-089 Warsaw, Poland.
² Biological and Chemical Research Centre, Faculty of Chemistry, University of Warsaw, Żwirki i Wigury 101, 02-089 Warsaw, Poland. Electronic address: kozmin@chem.uw.edu.pl.

PMID: 26592945
DOI: 10.1016/j.pnmrs.2015.07.001

Abstract

High dimensionality NMR experiments facilitate resonance assignment and precise determination of spectral parameters such as coupling constants. Sparse non-uniform sampling enables acquisition of experiments of high dimensionality with high resolution in acceptable time. In this review we present and compare some significant applications of NMR experiments of dimensionality higher than three in the field of biomolecular studies in solution.

Keywords: NMR spectroscopy; Non-uniform sampling; Proteins; RNA; Sparse sampling.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Humans
Nuclear Magnetic Resonance, Biomolecular / methods*
Proteins / chemistry
RNA / chemistry
Solutions

Substances

Proteins
Solutions
RNA