Oligonucleotide-Based Mimetics of Hepatocyte Growth Factor

Ryosuke Ueki; Ayaka Ueki; Naoto Kanda; Shinsuke Sando

doi:10.1002/anie.201508572

Oligonucleotide-Based Mimetics of Hepatocyte Growth Factor

Angew Chem Int Ed Engl. 2016 Jan 11;55(2):579-82. doi: 10.1002/anie.201508572. Epub 2015 Nov 23.

Authors

Ryosuke Ueki¹, Ayaka Ueki¹, Naoto Kanda¹, Shinsuke Sando²

Affiliations

¹ Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan).
² Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan). ssando@chembio.t.u-tokyo.ac.jp.

PMID: 26592704
DOI: 10.1002/anie.201508572

Abstract

Oligonucleotide-based hepatocyte growth factor (HGF) mimetics are described. A DNA aptamer to Met, a cognate receptor for HGF, was shown to induce Met activation when used in dimer form. The most potent aptamer dimer, ss-0, which was composed solely of 100-mer single-stranded DNA, exhibited nanomolar potency. Aptamer ss-0 reproduced HGF-induced cellular behaviors, including migration and proliferation. The present work sheds light on oligonucleotides as a novel chemical entity for the design of growth factor mimetics.

Keywords: DNA nanotechnology; aptamers; growth factors; receptors; signal transduction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Cell Line
Dimerization
Hepatocyte Growth Factor / chemistry*
Humans
Molecular Mimicry*
Oligonucleotides / chemistry*
Oligonucleotides / genetics
Phosphorylation
Proto-Oncogene Proteins c-met / genetics

Substances

Oligonucleotides
Hepatocyte Growth Factor
Proto-Oncogene Proteins c-met