Abstract
Newcastle disease virus (NDV) expressing HIV-1 BaL gp160 was evaluated either alone or with monomeric BaL gp120 and BaL SOSIP gp140 protein in a prime-boost combination in guinea pigs to enhance envelope (Env)-specific humoral and mucosal immune responses. We showed that a regimen consisting of an NDV prime followed by a protein boost elicited stronger serum and mucosal Th-1-biased IgG responses and neutralizing antibody responses than NDV-only immunizations. Additionally, these responses were higher after the gp120 than after the SOSIP gp140 protein boost.
Copyright © 2016, American Society for Microbiology. All Rights Reserved.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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AIDS Vaccines / administration & dosage*
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AIDS Vaccines / genetics
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AIDS Vaccines / immunology*
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Administration, Intranasal
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Animals
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Antibodies, Neutralizing / blood
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Drug Carriers
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Enzyme-Linked Immunosorbent Assay
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Female
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Genetic Vectors
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Guinea Pigs
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HIV Antibodies / blood
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HIV Envelope Protein gp120 / genetics
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HIV Envelope Protein gp120 / immunology*
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HIV Envelope Protein gp160 / genetics
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HIV Envelope Protein gp160 / immunology*
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Immunization Schedule*
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Immunoglobulin G / blood
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Injections, Intramuscular
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Neutralization Tests
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Newcastle disease virus / genetics
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Th1 Cells / immunology
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Vaccination / methods*
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Vaccines, Synthetic / administration & dosage
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
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env Gene Products, Human Immunodeficiency Virus / genetics
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env Gene Products, Human Immunodeficiency Virus / immunology*
Substances
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AIDS Vaccines
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Antibodies, Neutralizing
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Drug Carriers
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HIV Antibodies
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HIV Envelope Protein gp120
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HIV Envelope Protein gp160
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Immunoglobulin G
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Vaccines, Synthetic
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env Gene Products, Human Immunodeficiency Virus
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gp120 protein, Human immunodeficiency virus 1
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gp140 envelope protein, Human immunodeficiency virus 1
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gp160 protein, Human immunodeficiency virus 1