Isolation of an exopolysaccharide-producing heavy metal-resistant Halomonas sp. MG

Arch Microbiol. 2016 Mar;198(2):205-9. doi: 10.1007/s00203-015-1173-5. Epub 2015 Nov 18.

Abstract

An exopolysaccharide (EPS)-producing heavy metal-resistant Gram-negative bacterium was isolated from ore-contaminated soil. The selected strain was identified by 16S rDNA sequencing and designated as Halomonas sp. MG. Phylogenetic analysis of the gene sequence showed its close similarity with Halomonas sp. Field emission scanning electron microscopy analysis revealed that the EPS had a porous structure with small pores. X-ray diffractograms showed the non-crystalline nature of the EPS. Further, FTIR spectroscopic analysis revealed the presence of carboxyl, hydroxyl and amide groups corresponding to a typical EPS.

Keywords: Exopolysaccharide; Heavy metal; Ore; Polymer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Halomonas / classification*
  • Halomonas / drug effects
  • Halomonas / isolation & purification*
  • Halomonas / metabolism
  • Metals, Heavy / toxicity
  • Microscopy, Electron, Scanning
  • Phylogeny
  • Polysaccharides, Bacterial / metabolism*
  • Polysaccharides, Bacterial / ultrastructure
  • RNA, Ribosomal, 16S / genetics
  • Species Specificity

Substances

  • Metals, Heavy
  • Polysaccharides, Bacterial
  • RNA, Ribosomal, 16S