A coherent transcriptional feed-forward motif model for mediating auxin-sensitive PIN3 expression during lateral root development

Qian Chen; Yang Liu; Steven Maere; Eunkyoung Lee; Gert Van Isterdael; Zidian Xie; Wei Xuan; Jessica Lucas; Valya Vassileva; Saeko Kitakura; Peter Marhavý; Krzysztof Wabnik; Niko Geldner; Eva Benková; Jie Le; Hidehiro Fukaki; Erich Grotewold; Chuanyou Li; Jiří Friml; Fred Sack; Tom Beeckman; Steffen Vanneste

doi:10.1038/ncomms9821

A coherent transcriptional feed-forward motif model for mediating auxin-sensitive PIN3 expression during lateral root development

Nat Commun. 2015 Nov 18:6:8821. doi: 10.1038/ncomms9821.

Authors

Qian Chen^{1

2

3}, Yang Liu^{1

2

4}, Steven Maere^{1

2}, Eunkyoung Lee⁵, Gert Van Isterdael^{1

2}, Zidian Xie⁶, Wei Xuan^{1

2}, Jessica Lucas⁵, Valya Vassileva^{1

2

7}, Saeko Kitakura^{1

2

8}, Peter Marhavý^{9

10}, Krzysztof Wabnik^{1

2

9}, Niko Geldner¹⁰, Eva Benková⁹, Jie Le¹¹, Hidehiro Fukaki¹², Erich Grotewold⁶, Chuanyou Li³, Jiří Friml^{1

2

9}, Fred Sack⁵, Tom Beeckman^{1

2}, Steffen Vanneste^{1

2}

Affiliations

¹ Department of Plant Systems Biology, VIB, Ghent 9052, Belgium.
² Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.
³ State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
⁴ Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
⁵ Department of Botany, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
⁶ Center for Applied Plant Sciences (CAPS) and Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, USA.
⁷ Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Academik Georgi Bonchev Street, Sofia 1113, Bulgaria.
⁸ Department of Biological Sciences, Graduate School of Science, Osaka University, Osaka 560-0043 Japan.
⁹ Institute of Science and Technology Austria, Klosterneuburg 3400, Austria.
¹⁰ Department of Plant Molecular Biology, UNIL-Sorge, University of Lausanne, Lausanne 1015, Switzerland.
¹¹ Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
¹² Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai, Kobe 657-8501, Japan.

Abstract

Multiple plant developmental processes, such as lateral root development, depend on auxin distribution patterns that are in part generated by the PIN-formed family of auxin-efflux transporters. Here we propose that AUXIN RESPONSE FACTOR7 (ARF7) and the ARF7-regulated FOUR LIPS/MYB124 (FLP) transcription factors jointly form a coherent feed-forward motif that mediates the auxin-responsive PIN3 transcription in planta to steer the early steps of lateral root formation. This regulatory mechanism might endow the PIN3 circuitry with a temporal 'memory' of auxin stimuli, potentially maintaining and enhancing the robustness of the auxin flux directionality during lateral root development. The cooperative action between canonical auxin signalling and other transcription factors might constitute a general mechanism by which transcriptional auxin-sensitivity can be regulated at a tissue-specific level.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Arabidopsis / genetics*
Arabidopsis / growth & development
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Chromatin Immunoprecipitation
Feedback, Physiological
Gene Expression Regulation, Plant*
Glucuronidase / metabolism
Organisms, Genetically Modified
Plant Roots / growth & development*
Plant Roots / metabolism
RNA, Messenger / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Transcription Factors / genetics*
Transcription Factors / metabolism
Transcription, Genetic

Substances

ARF7 protein, Arabidopsis
Arabidopsis Proteins
FLP protein, Arabidopsis
PIN3 protein, Arabidopsis
RNA, Messenger
Transcription Factors
Glucuronidase