This study analyzed the association between self-reported diagnosis of hearing loss and individual and occupational factors among urban transportation workers in Greater Metropolitan Belo Horizonte, Minas Gerais State, Brazil. The sample size was calculated by quotas and stratified by occupation (drivers and fare collectors) in the urban transportation companies in Belo Horizonte, Betim, and Contagem. Data were collected with face-to-face interviews and recorded by the interviewers on netbooks. The dependent variable was defined as an affirmative response to the question on prevailing medical diagnosis of hearing loss. The independent variables were organized in three blocks: social and demographic characteristics, lifestyle, and work aspects. Diagnosis of hearing loss was reported by 213 of the 1,527 workers and was associated with age and diagnosis of tinnitus. At the occupational level, hearing loss was associated with history of sick leave, time-on-the-job, and two environmental risks, unbearable noise and whole-body vibration. Measures to prevent hearing loss are needed for urban transportation workers.