Simultaneous Determination of Plant Hormones by GC-TOF-MS

José G Vallarino; Sonia Osorio

doi:10.1007/978-1-4939-3115-6_19

Simultaneous Determination of Plant Hormones by GC-TOF-MS

Methods Mol Biol. 2016:1363:229-37. doi: 10.1007/978-1-4939-3115-6_19.

Authors

José G Vallarino¹, Sonia Osorio²

Affiliations

¹ Departamento de Biología Molecular y Bioquímica, Instituto de Hortofruticultura Subtropical y Mediterranea (IHSM), Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Campus Universitario de Teatinos, Málaga, E-29071, Spain.
² Departamento de Biología Molecular y Bioquímica, Instituto de Hortofruticultura Subtropical y Mediterranea (IHSM), Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Campus Universitario de Teatinos, Málaga, E-29071, Spain. sosorio@uma.es.

PMID: 26577794
DOI: 10.1007/978-1-4939-3115-6_19

Abstract

Phytohormones are key signaling molecules that coordinate plant growth and development through a range of complex interactions. Since the vast majority of plant responses to given stimuli result, amongst other factors, from a crosstalk between hormones, simultaneous analysis of multiple hormones is vital to improve our understanding of these interactions. This chapter describes a sensitive, reliable, and inexpensive method for quantification of multiple phytohormones by gas chromatography-mass spectrometry (GC-MS).

Keywords: Abscisic acid; Derivatization; GC -MS; Hormones; Indole-3-acetic acid; Jasmonic acid; Plant tissue; Salicylic acid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gas Chromatography-Mass Spectrometry* / methods
Plant Growth Regulators / analysis*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* / methods

Substances

Plant Growth Regulators