Genome Analysis of Kingella kingae Strain KWG1 Reveals How a β-Lactamase Gene Inserted in the Chromosome of This Species

Antimicrob Agents Chemother. 2015 Nov 16;60(1):703-8. doi: 10.1128/AAC.02192-15. Print 2016 Jan.

Abstract

We describe the genome of a penicillinase-producing Kingella kingae strain (KWG1), the first to be isolated in continental Europe, whose bla(TEM-1) gene was, for the first time in this species, found to be chromosomally inserted. The bla(TEM) gene is located in an integrative and conjugative element (ICE) inserted in Met-tRNA and comprising genes that encode resistance to sulfonamides, streptomycin, and tetracycline. This ICE is homologous to resistance-conferring plasmids of K. kingae and other Gram-negative bacteria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Chromosome Mapping
  • Chromosomes, Bacterial / chemistry*
  • Drug Resistance, Bacterial / genetics
  • Gene Expression Regulation, Bacterial*
  • Gene Ontology
  • Genome, Bacterial*
  • Humans
  • Kingella kingae / drug effects
  • Kingella kingae / genetics*
  • Kingella kingae / isolation & purification
  • Kingella kingae / metabolism
  • Molecular Sequence Annotation
  • Neisseriaceae Infections / microbiology
  • Plasmids / chemistry
  • Plasmids / metabolism
  • Streptomycin / pharmacology
  • Sulfonamides / pharmacology
  • Tetracycline / pharmacology
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Sulfonamides
  • beta-Lactamases
  • Tetracycline
  • Streptomycin