The Isolation, Identification and Analyses of Lactobacillus Genus Bacteria with Probiotic Potential

Adv Exp Med Biol. 2016:897:103-11. doi: 10.1007/5584_2015_5008.

Abstract

103 strains of lactic acid bacteria of Lactobacillus genus were isolated from natural sources and identified for genus and species level with API tests and 16S rRNA sequencing. However, only 27 strains from isolated cultures demonstrated a high stability to gastric stress and from that - only 15 strains were highly resistant to intestinal stress. Results indicated that only some isolated cultures of lactobacilli possessed potential probiotical properties and could serve as new probiotics for dairy industry with high resistance to gastro-intestinal stresses.

Keywords: Gastro-intestinal stress; Isolation of lactic acid bacteria; Molecular identification; Probiotic properties.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dairying
  • Food Microbiology
  • Humans
  • Lactobacillus* / classification
  • Lactobacillus* / genetics
  • Lactobacillus* / growth & development
  • Lactobacillus* / isolation & purification
  • Probiotics* / classification
  • Probiotics* / isolation & purification
  • RNA, Bacterial / genetics*
  • RNA, Ribosomal, 16S / genetics*
  • Stress, Physiological*

Substances

  • RNA, Bacterial
  • RNA, Ribosomal, 16S