Raman spectroscopy-based identification of nosocomial outbreaks of the clonal bacterium Escherichia coli

J G Kusters; W B van Leeuwen; K Maquelin; H E M Blok; H F M Willemse; L A M de Graaf-Miltenburg; A C Fluit; A Troelstra

doi:10.1007/s10096-015-2511-x

Raman spectroscopy-based identification of nosocomial outbreaks of the clonal bacterium Escherichia coli

Eur J Clin Microbiol Infect Dis. 2016 Jan;35(1):83-7. doi: 10.1007/s10096-015-2511-x. Epub 2015 Nov 12.

Authors

J G Kusters¹, W B van Leeuwen², K Maquelin³, H E M Blok³, H F M Willemse⁴, L A M de Graaf-Miltenburg³, A C Fluit³, A Troelstra³

Affiliations

¹ Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands. h.kusters@umcutrecht.nl.
² Department of Innovative Molecular Diagnostics, University of Applied Sciences Leiden, Leiden, The Netherlands.
³ Center for Optical Diagnostics and Therapy, Department of Dermatology, Erasmus University Medical Center, Rotterdam, The Netherlands.
⁴ Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands.

Abstract

DNA-based techniques are frequently used to confirm the relatedness of putative outbreak isolates. These techniques often lack the discriminatory power when analyzing closely related microbes such as E. coli. Here the value of Raman spectroscopy as a typing tool for E. coli in a clinical setting was retrospectively evaluated.

Publication types

Evaluation Study

MeSH terms

Bacterial Typing Techniques / methods*
Cross Infection / epidemiology*
Cross Infection / microbiology
Disease Outbreaks*
Escherichia coli / chemistry
Escherichia coli / classification*
Escherichia coli / isolation & purification
Escherichia coli Infections / epidemiology*
Escherichia coli Infections / microbiology
Humans
Retrospective Studies
Spectrum Analysis, Raman / methods*