Construction, Enzyme Response, and Substrate Capacity of a Hyaluronan-Cyclodextrin Supramolecular Assembly

Chem Asian J. 2016 Feb 18;11(4):505-11. doi: 10.1002/asia.201501029. Epub 2015 Dec 10.

Abstract

A supramolecular assembly was constructed with a cationic cyclodextrin (EICD) and native hyaluronan (HA). The cationic carboxylic ester pendants on HA support hyaluronidase (HAase)-responsive sites and the EICD supports artificial carboxylic esterase responsive sites. Substrate-binding models were investigated by using environment-sensitive fluorescence probes 2-p-toluidino-6-naphthalenesulfoniate sodium (2,6-TNS) and thioflavin T (ThT). On a HA/EICD assembly, EICD was able to bind an anionic substrate and HA and EICD constructed the cationic substrate binding site together. This assembly could be used as a sequential dual-substrate carrier.

Keywords: cyclodextrins; enzymes; fluorescent probes; substrate binding; supramolecular chemistry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carboxylesterase / metabolism*
  • Cattle
  • Cyclodextrins / chemistry
  • Cyclodextrins / metabolism*
  • Delayed-Action Preparations / chemistry
  • Delayed-Action Preparations / metabolism*
  • Hyaluronic Acid / chemistry
  • Hyaluronic Acid / metabolism*
  • Hyaluronoglucosaminidase / metabolism*
  • Kinetics

Substances

  • Cyclodextrins
  • Delayed-Action Preparations
  • Hyaluronic Acid
  • Carboxylesterase
  • Hyaluronoglucosaminidase