Azole Resistance in Aspergillus fumigatus Clinical Isolates from an Italian Culture Collection

Antimicrob Agents Chemother. 2015 Nov 9;60(1):682-5. doi: 10.1128/AAC.02234-15. Print 2016 Jan.

Abstract

The aims of the study were to investigate the prevalence of azole resistance among Aspergillus fumigatus clinical isolates. A total of 533 clinical isolates that had been collected between 1995 and 2006, from 441 patients, were screened. No resistance was detected in isolates collected between 1995 and 1997. Starting in 1998, the resistance rate was 6.9%; a total of 24 patients (6.25%) harbored a resistant isolate. The TR34/L98H substitution was found in 21 of 30 tested isolates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Antifungal Agents / pharmacology*
  • Aspergillosis / drug therapy
  • Aspergillosis / epidemiology*
  • Aspergillosis / microbiology
  • Aspergillus fumigatus / drug effects
  • Aspergillus fumigatus / enzymology
  • Aspergillus fumigatus / genetics*
  • Aspergillus fumigatus / isolation & purification
  • Bacterial Typing Techniques
  • Cytochrome P-450 Enzyme System / genetics*
  • Cytochrome P-450 Enzyme System / metabolism
  • Drug Resistance, Fungal / genetics*
  • Epidemiological Monitoring
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Expression
  • Genotype
  • Humans
  • Italy / epidemiology
  • Itraconazole / pharmacology
  • Microbial Sensitivity Tests
  • Microsatellite Repeats
  • Mutation
  • Promoter Regions, Genetic
  • Triazoles / pharmacology
  • Voriconazole / pharmacology

Substances

  • Antifungal Agents
  • Fungal Proteins
  • Triazoles
  • Itraconazole
  • posaconazole
  • Cytochrome P-450 Enzyme System
  • cytochrome P-450 CYP51A, Aspergillus
  • Voriconazole

Grants and funding

This study was partially supported by the Gilead Fellowship Program 2015.