Aptamer-Based Enzyme Capture Assay for Measurement of Plasma Thrombin Levels

Jens Müller; Tobias Becher; Günter Mayer; Bernd Pötzsch

doi:10.1007/978-1-4939-3197-2_15

Aptamer-Based Enzyme Capture Assay for Measurement of Plasma Thrombin Levels

Methods Mol Biol. 2016:1380:179-89. doi: 10.1007/978-1-4939-3197-2_15.

Authors

Jens Müller¹, Tobias Becher², Günter Mayer³, Bernd Pötzsch²

Affiliations

¹ Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany. jens.mueller@ukb.uni-bonn.de.
² Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany.
³ Life & Medical Sciences Institute (LIMES), University of Bonn, Bonn, Germany.

PMID: 26552826
DOI: 10.1007/978-1-4939-3197-2_15

Abstract

The quantification of circulating thrombin is a valuable tool to accurately assess the activity of the blood coagulation system. Here, we describe the combined application of the thrombin-specific reversible active-site inhibitor argatroban and the DNA-aptamer HD1-22 for conduction of an enzyme capture assay for reliable measurement of plasma thrombin levels.

Keywords: Active-site inhibitor; Aptamer; Argatroban; Enzyme capture assay; Plasma; Thrombin.

MeSH terms

Antithrombins / pharmacology
Aptamers, Nucleotide* / metabolism
Arginine / analogs & derivatives
Enzyme Assays / methods*
Humans
Pipecolic Acids / pharmacology
Sulfonamides
Thrombin* / antagonists & inhibitors
Thrombin* / metabolism

Substances

Antithrombins
Aptamers, Nucleotide
Pipecolic Acids
Sulfonamides
Arginine
Thrombin
argatroban