In Vitro and In Vivo Imaging of Fluorescent Aptamers

Methods Mol Biol. 2016:1380:135-50. doi: 10.1007/978-1-4939-3197-2_11.

Abstract

Fluorescence imaging techniques could be used in different ways to study the interaction of aptamers with biological systems from cell culture to animal models. Here, we present the methods developed in our laboratory for fluorescently labeled aptamers, study their internalization inside living cells using time-lapse microscopy, and monitor their biodistribution in mice bearing subcutaneous xenograft tumors using planar fluorescence imaging and fluorescence diffuse optical tomography (fDOT).

Keywords: Aptamer; Fluorescence; Fluorescence diffuse optical tomography; Oligonucleotide; Time-lapse microscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aptamers, Nucleotide* / chemistry
  • Aptamers, Nucleotide* / metabolism
  • Cell Line, Tumor
  • Disease Models, Animal
  • Fluorescent Dyes* / chemistry
  • Heterografts
  • Humans
  • Imaging, Three-Dimensional / methods
  • In Vitro Techniques
  • Mice
  • Mice, Nude
  • Microscopy, Video / methods
  • Molecular Imaging / methods*
  • Neoplasms / diagnosis
  • Neoplasms / metabolism
  • Staining and Labeling
  • Time-Lapse Imaging / methods
  • Tomography, Optical / methods

Substances

  • Aptamers, Nucleotide
  • Fluorescent Dyes