Applications of Aptamers in Flow and Imaging Cytometry

Isis C Nascimento; Arthur A Nery; Vinícius Bassaneze; José E Krieger; Henning Ulrich

doi:10.1007/978-1-4939-3197-2_10

Applications of Aptamers in Flow and Imaging Cytometry

Methods Mol Biol. 2016:1380:127-34. doi: 10.1007/978-1-4939-3197-2_10.

Authors

Isis C Nascimento¹, Arthur A Nery¹, Vinícius Bassaneze², José E Krieger², Henning Ulrich³

Affiliations

¹ Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, São Paulo, 05508-900, Brazil.
² Laboratory of Genetics and Molecular Cardiology/LIM 13, Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil.
³ Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, São Paulo, 05508-900, Brazil. henning@iq.usp.br.

PMID: 26552821
DOI: 10.1007/978-1-4939-3197-2_10

Abstract

Aptamers compete with antibodies in many applications, in which high-affinity and specificity ligands are needed. In this regard, fluorescence-tagged aptamers have gained applications in flow and imaging cytometry for detecting cells expressing distinct antigens. Here we present prospective methods, as a starting point, for using these high-affinity ligands for cytometry applications.

Keywords: Aptamers; Automation; Flow cytometry; Fluorescence-labeling; Imaging cytometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / chemistry
Antibodies, Monoclonal / metabolism
Aptamers, Nucleotide* / chemistry
Aptamers, Nucleotide* / metabolism
Flow Cytometry / methods*
Image Cytometry / methods*
Kinetics
Protein Binding
Staining and Labeling

Substances

Antibodies, Monoclonal
Aptamers, Nucleotide