Independent Control of Topography for 3D Patterning of the ECM Microenvironment

Adv Mater. 2016 Jan 6;28(1):132-7. doi: 10.1002/adma.201503950. Epub 2015 Nov 9.

Abstract

Biomimetic extracellular matrix (ECM) topographies driven by the magnetic-field-directed self-assembly of ECM protein-coated magnetic beads are fabricated. This novel bottom-up method allows us to program isotropic, anisotropic, and diverse hybrid ECM patterns without changing other physicochemical properties of the scaffold material. It is demonstrated that this 3D anisotropic matrix is able to guide the dendritic protrusion of cells.

Keywords: 3D biomaterials; biomimetic extracellular matrix; extracellular matrix topography; magnetic-field-directed self-assembly.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / pharmacology*
  • Cell Proliferation / drug effects
  • Cellular Microenvironment / drug effects*
  • Extracellular Matrix / drug effects*
  • Extracellular Matrix / metabolism
  • Magnetic Fields
  • Mice
  • NIH 3T3 Cells
  • PC12 Cells
  • Rats
  • Tissue Scaffolds / chemistry*

Substances

  • Biocompatible Materials