Separation and purification of two new and two known alkaloids from leaves of Nitraria sibirica by pH-zone-refining counter-current chromatography

Mahinur Bakri; Qibin Chen; Qingling Ma; Yi Yang; Abdumijit Abdukadir; Haji Akber Aisa

doi:10.1016/j.jchromb.2015.10.038

Separation and purification of two new and two known alkaloids from leaves of Nitraria sibirica by pH-zone-refining counter-current chromatography

J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Dec 1:1006:138-145. doi: 10.1016/j.jchromb.2015.10.038. Epub 2015 Nov 11.

Authors

Mahinur Bakri¹, Qibin Chen², Qingling Ma¹, Yi Yang¹, Abdumijit Abdukadir¹, Haji Akber Aisa³

Affiliations

¹ The Key Laboratory of Plant Resources and Chemistry in Arid Regions and Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, China.
² The Key Laboratory of Plant Resources and Chemistry in Arid Regions and Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, China; University of Chinese Academy of Sciences, Beijing, China.
³ The Key Laboratory of Plant Resources and Chemistry in Arid Regions and Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, China. Electronic address: haji@ms.xjb.ac.cn.

PMID: 26551205
DOI: 10.1016/j.jchromb.2015.10.038

Abstract

The total alkaloids from Nitraria sibirica leaves have been confirmed to exhibit significant protective effects against inflammatory renal injury, hypertension and albuminuria in angiotensin II-salt hypertension. In the present study, a separation method of pH-zone-refining counter-current chromatography was established for separation of the alkaloids from N. sibirica. The separation was performed with a solvent system of MtBE-n-BuOH-H2O (2:2:5, v/v) at a flow rate of 2.0mL/min. And 15mM triethylamine (TEA) was added to the upper organic phase, while 10mM hydrochloric acid was added to the lower aqueous phase. As a result, a new alkaloid, schobemine (5.6mg), and a known alkaloid, nitraramine (5.0mg), together with fractions A and B were obtained from the total alkaloids of N. sibirica. The fractions A and B were further purified by means of pH-zone-refining counter-current chromatography with solvent systems of n-hexane-n-BuOH-H2O (1.5:3.5:5, v/v) and (2:3:5, v/v), respectively. TEA (10mM) was added to the upper phase, and 10mM of HCl was added to the lower phase in above two solvent systems, respectively. As a result, a known alkaloid, schoberidine (5.0mg), and a new alkaloid, schoberimine (3.0mg) were obtained from fractions A and B, respectively. The purities of the compounds were measured by HPLC-ELSD, and their structures were identified by ESI-MS, 1D and 2D NMR.

Keywords: Alkaloids; Novel compounds; Separation and purification; pH-zone-refining counter-current chromatography.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkaloids / analysis
Alkaloids / isolation & purification*
Chromatography, Thin Layer
Countercurrent Distribution / methods*
Magnoliopsida / chemistry*
Models, Molecular
Plant Leaves / chemistry*

Substances

Alkaloids