Quantitative MS analysis of therapeutic mAbs and their glycosylation for pharmacokinetics study

Proteomics Clin Appl. 2016 Apr;10(4):303-14. doi: 10.1002/prca.201500098. Epub 2015 Dec 10.

Abstract

Therapeutic mAbs play an important role in the treatment of a wide range of diseases. Due to their complexity, comprehensive evaluation of their pharmacokinetics has yet to be fully achieved. It is crucial to develop sensitive, accurate, reliable, and reproducible methods for quantitation of mAbs in complex samples. In addition, it is also important to evaluate the PTMs which can affect their safety and/or effectiveness. MS-based methods provide an emerging approach for quantitation of proteins and their modification forms. In this review, we give a brief overview of quantification analysis of mAbs in complex biological samples and the characterization of N-glycosylation by biological MS.

Keywords: Mass spectrometry; Pharmacokinetics; Proteomics; Therapeutic mAb.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Antibodies, Monoclonal / analysis*
  • Antibodies, Monoclonal / pharmacokinetics
  • Antibodies, Monoclonal / therapeutic use
  • Chromatography, Liquid
  • Glycosylation
  • Half-Life
  • Humans
  • Immunoglobulin Fab Fragments / analysis*
  • Immunoglobulin Fc Fragments / analysis*
  • Isotope Labeling
  • Mass Spectrometry
  • Peptide Fragments / analysis*
  • Polysaccharides / analysis*
  • Protein Processing, Post-Translational*
  • Proteolysis
  • Trypsin / chemistry

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin Fab Fragments
  • Immunoglobulin Fc Fragments
  • Peptide Fragments
  • Polysaccharides
  • Trypsin