High-throughput sexing of mouse blastocysts by real-time PCR using dissociation curves

Andrew M Prantner; Teri Ord; Sergey Medvedev; George L Gerton

doi:10.1002/mrd.22595

High-throughput sexing of mouse blastocysts by real-time PCR using dissociation curves

Mol Reprod Dev. 2016 Jan;83(1):6-7. doi: 10.1002/mrd.22595. Epub 2015 Nov 23.

Authors

Andrew M Prantner¹, Teri Ord¹, Sergey Medvedev¹, George L Gerton^{1

2

3}

Affiliations

¹ Center for Research on Reproduction and Women's Health Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.
² Center of Excellence in Environmental Toxicology Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.
³ Department of Obstetrics and Gynecology Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.

Abstract

Sex ratio is defined as the proportion of males to females in a population. Subdivisions of the sex ratio are the primary (ratio at fertilization) and the secondary (ratio at birth). The expected secondary sex ratio is 0.5, but biological, environmental, or occupational variables can shift the secondary sex ratio from this expectation.

Publication types

Letter
Research Support, N.I.H., Extramural

MeSH terms

Animals
Blastocyst / cytology*
Blastocyst / metabolism*
Data Interpretation, Statistical
Female
High-Throughput Screening Assays* / methods
Male
Mice
Real-Time Polymerase Chain Reaction* / methods
Sex Determination Analysis / methods*
Sex Differentiation / physiology
Sex Ratio

Abstract

Publication types

MeSH terms

Grants and funding